鳜鱼 ( Siniperca chuatsi))是一种受欢迎的内陆养殖鱼类，具有很高的经济价值。迫切需要分子选择性育种计划来改善养殖鳜鱼的生长性状。在这项研究中，我们使用 160 条鳜鱼个体、特定位点扩增片段测序和 SNP 基因分型，进行了全基因组关联研究 (GWAS)，以确定可能与鳜鱼各种生长性状相关的基因组区域和候选基因，例如体重（BW）、体厚（BT）、体高（BH）、总身长（TL）、屠宰体重（SW）和体长（BL）。通过基因分型，在24条染色体上发现了5205个高质量的SNP。共获得11个与生长性状相关的显着SNP和37个暗示SNP，其中与BW显着相关的5个SNP位于连锁群（LG）8、17、21和23，与TL显着相关的2个SNP位于LG 7和17，与BH显着相关的2个SNP位于LG如图17和23所示，两个与BT显着相关的SNP分别位于LG 14和19。此外，LG17_937,734 和 LG23_23,856,613 是与生长相关的性状重叠。在这些重要 SNP 上游和下游 300 kb 内，在相关 QTL 中鉴定出 65 个候选基因，其中许多与骨分化、生长和发育、细胞分裂和神经发生有关。特别是，与 BT 显着相关的两个 SNP 分别位于 LG 14 和 19。此外，LG17_937,734 和 LG23_23,856,613 是与生长相关的性状重叠。在这些重要 SNP 上游和下游 300 kb 内，在相关 QTL 中鉴定出 65 个候选基因，其中许多与骨分化、生长和发育、细胞分裂和神经发生有关。特别是，与 BT 显着相关的两个 SNP 分别位于 LG 14 和 19。此外，LG17_937,734 和 LG23_23,856,613 是与生长相关的性状重叠。在这些重要 SNP 上游和下游 300 kb 内，在相关 QTL 中鉴定出 65 个候选基因，其中许多与骨分化、生长和发育、细胞分裂和神经发生有关。特别是，ASNS，TG，MKK6，HTRA1，RNF213，TTN，TGFBR2，和NCK2，显示出与生长，脂肪，肌肉和骨形成密切相关。据我们所知，这是第一个关注鳜鱼生长特性的 GWAS。我们的研究结果为分子标记辅助选择、基因组选择和鳜鱼生长特性的功能分析奠定了基础。
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Genome-wide association study reveals growth-related SNPs and candidate genes in mandarin fish (Siniperca chuatsi)
Mandarin fish (Siniperca chuatsi) is a popular inland farmed fish species with high an economic value. Molecular selective breeding programs are urgently required to improve the growth traits of mandarin fish for farming industry. In this study, using 160 mandarin fish individuals, specific locus-amplified fragment sequencing and SNP genotyping, we performed a genome-wide association study (GWAS) to identify genomic regions and candidate genes that are potentially associated with various growth traits of mandarin fish, such as body weight (BW), body thickness (BT), body height (BH) total length (TL), slaughter weight (SW), and body length (BL). Through genotyping, 5205 high-quality SNPs were found located on 24 chromosomes. A total of 11 significant and 37 suggestive SNPs related to growth traits were obtained, among which five SNPs significantly related to BW were located in the linkage group (LG) 8, 17, 21, and 23, two SNPs significantly related to TL were located in LG 7 and 17, two SNPs significantly related to BH were located on LG 17 and 23, two SNPs significantly related to BT were located on LG 14 and 19, respectively. Furthermore, LG17_937,734 and LG23_23,856,613 were growth-related traits overlap. Within 300 kb upstream and downstream of these significant SNPs, 65 candidate genes were identified within the associated QTLs, many of which were related to bone differentiation, growth and development, cell division and neurogenesis. In particular, asns, tg, mkk6, htra1, rnf213, ttn, tgfbr2, and nck2, were shown to be closely associated with growth, fat, muscle and bone formation. To our knowledge, this is the first GWAS focusing on growth traits of mandarin fish. Our findings establish a foundation for molecular marker-assisted selecting, genomic selecting, and functional analyzing the growth traits of mandarin fish.